Modifying Enzymes
New England Biolabs, Inc. Exonuclease I Reaction Buffer – 6 ml
This is the NEB reaction buffer supplied with Exonuclease I. New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
LifeSensors SUMOSTAR PROTEASE 1000 UNITS
Recombinant SUMOstar Protease 1 specifically recognizes and cleaves the SUMOstar tag from recombinant fusion proteins made with SUMOstar expression systems (Cat.#s: 1100A, 1106, 1100K, 1101, 2100, 2101, 2160, 2161, 3100, 3101, 7110, 7111, 7120, 7121). Unlike thrombin, EK, or TEV proteases, which recognize short, linear sequences, SUMOstar Protease 1 recognizes the tertiary structure of SUMOstar. As a result, SUMOstar Protease 1 will not cleave within the protein of interest. SUMOstar Protease 1 will not cleave SUMO-tags from proteins expressed with the SUMOpro or SUMOpro3 systems. To cleave the SUMOpro tag (orginial yeast SUMO tag), please use SUMO Protease 1 and to cleave SUMOpro3 tag please use SUMO Protease 2. This product contains 1000 units of SUMOstar Protease, where 1 U will cleave >90 µg of SUMOstar-GFP in 1 hour at 37C
Sigma Aldrich Fine Chemicals Biosciences Cellulase from Trichoderma reesei aqueous solution, ≥700 units/g | 9012-54-8 | MFCD00081510 | 50mg
Cellulase from Trichoderma reesei aqueous solution, ≥700 units/g | 9012-54-8 | MFCD00081510 | 50mg
Bioworld alpha-Chymotrypsin, 250 mg
Specification:Grade: USP gradeForm: White crystalline powderChymotrypsin acitvity (dry basis): 1000 U/mgTrypsin: 1%Residue after ignition: 2.5%Loss on drying: 5%Solubility (water, 10,000 U/ml): Pass
New England Biolabs, Inc. Vaccinia Capping System – 400 units
Vaccinia Capping System provides the necessary components to add 7-methylguanylate cap structures (Cap 0) to the 5' end of RNA. In eukaryotes, these terminal cap structures are involved in stabilization, transport, and translation of mRNAs. Enzymatic production of capped RNA is an easy way to improve the stability and translational competence of RNA used for in vitro translation, transfection, and microinjection. Alternatively, use of labeled GTP in a reaction provides a convenient way to label any RNA containing a 5' terminal triphosphate. Vaccinia capping enzyme is composed of two subunits (D1 and D12). The D1 subunit carries three enzymatic activities (RNA triphosphatase, guanylyltransferase and guanine methyltransferase); all necessary for addition of a complete Cap 0 structure, m7Gppp5'N to 5' triphosphate RNA. In vitro transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and the methyl donor, SAM.
Enzo Life Sciences Cathepsin D (human liver), (purified) (25 µg)
Purity: ≥95% (SDS-PAGE). Formulation: Lyophilized with phosphate buffer. Source: Isolated from human liver. Long Term Storage: -80°C.
Zymo Research Corporation 5-hmC Glucosyltransferase, 100 U
Glucosylation of 5-hydroxymethylcytosine by 5hmC Glucosyltransferase can be used for sequence specific (see Cat. Nos. D5410 & D5411), locus specific, as well as global quantification of 5- hydroxymethylcytosine.
Zymo Research Corporation 5-hmC Glucosyltransferase, 200 U
Glucosylation of 5-hydroxymethylcytosine by 5hmC Glucosyltransferase can be used for sequence specific (see Cat. Nos. D5410 & D5411), locus specific, as well as global quantification of 5- hydroxymethylcytosine.
ACROBiosystems GENIUS™Nuclease DMF Filed
GENIUS™Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-8 bases in length.
ACROBiosystems Endoglycosidase H (Endo H) is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli. Endo H cleaves the chitobiose core of high-mannose oligosaccharides and a limited number of hybrid oligosaccharides from
Endoglycosidase H is a recombinant glycosidase which cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins. It is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli.
INTACT GENOMICS INC RNase Inhibitor, Murine (Glycerol-free) 20,000 Units
Intact Genomics (ig) Glycerol-free RNase Inhibitor is a 49.8 kDa recombinant protein of murine origin which inhibits RNases A, B and C by binding noncovalently in a 1:1 ratio with high affinity. Moreover, it does not inhibit polymerase activity when used with Taq DNA Polymerase, Reverse Transcriptase. Glycerol-free RNase Inhibitor from Murine is resistant to oxidation compared to the human/porcine RNase inhibitors as it lacks a pair of cysteine residues found in the human/porcine versions. Stable at less than 1 mM DTT.Benefits of Glycerol-Free Enzyme: Optimal for Lyophilization ensuring stability and performance for point-of-care tests like molecular diagnostics. Better Compatibility: Avoids interference. Improved Performance: Enzyme formulations are less viscous. Simplified Integration: Allows for more flexible formulation.Applications: cDNA synthesis RT-PCR/RT-qPCR In vitro transcription RNA labeling Microfluidics assays
Sigma Aldrich Fine Chemicals Biosciences Hyaluronidase from Sheep Testes | 37326-33-3 | MFCD00131351 | 500mg
Hyaluronidase from Sheep Testes | 37326-33-3 | MFCD00131351 | 500mg
New England Biolabs, Inc. T7 Exonuclease – 5000 units
T7 Exonuclease acts in the 5' to 3' direction, catalyzing the removal of 5' mononucleotides from linear or nicked duplex DNA.