Modifying Enzymes
INTACT GENOMICS INC Exonuclease III 10000 Units (100 units/µl)
Exonuclease III (Exo III) is an exodeoxyribonuclease that digests one strand of duplex DNA from a blunt end, 5′ overhang or nick. The enzyme acts in a 3′→5′ direction producing stretches of single-stranded DNA (1, 2). Exo III is not active on 3′ overhangs of 4 or more bases in length and ssDNA. The enzyme has also RNase H, 3′-DNA phosphatase, and apurinic DNA endonuclease activities. Exo III digestions proceed at a uniform rate yielding predictable and reproducible results. However, the observed rate of nucleotide excision can vary depending on the individual characteristics of the reaction mix such as reaction temperature, ionic strength, template base content, template helical structure, and enzyme-to-DNA ratios (3-5).Applications• 3′ → 5′ exonuclease• Production of unidirectional nested deletions• Production of intermediates for site-directed mutagenesis• Preparation of strand-specific probes• Preparation of single-stranded substrates for dideoxy sequencing
Aldevron Dnase I, 70ug
70ug dispense of Dnase I. DNase I nonspecifically cleaves single- or double-stranded DNA, producing di-, tri- and oligonucleotide products. DNase I is free of detectable ribonuclease activity.
Qiagen Beverly Inc T4 RNA LIGASE 2
RNA Ligase; T4 RNA Ligase 2; Conc. 30,000 U/ul; 4,500 U; incl. 10X buffer
American Research Products Inc BoVine DeoXyribonuclease 1 DN
BoVine DeoXyribonuclease-1 (DNASE1) ELISA kit from CUSABIO Detection Range 12 5 pg/mL-800 pg/mL Sensitivity 3 1 pg/mL Sample serum plasma tissue homogenates Method Sandwich
Medchemexpress LLC DNA Ligase IV antibody (YA2699) | 10 UL
DNA Ligase IV antibody (YA2699) is a rabbit-derived, non-conjugated IgG monoclonal antibody that targets DNA Ligase IV. This antibody plays a critical role in DNA non-homologous end joining (NHEJ), specifically in double-strand break (DSB) repair and V(D)J recombination, by catalyzing the NHEJ ligation step to reseal DNA breaks.
- Rabbit-derived IgG monoclonal antibody
- Non-conjugated
- Targets DNA Ligase IV
- Reactivity: Human
- Application: WB (Western Blot)
- Dilution ratio: 1:500-1:1000 for WB
- SwissProt ID: P49917
- Research field: Epigenetics and nuclear signaling
- Supplied in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
- Appearance: Liquid
- Storage: Stored at -20°C for 1 year, avoid repeated freeze/thaw cycles
INTACT GENOMICS INC Cas9 Nuclease 40 µg
Intact Genomics Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing (1, 2). The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie blue staining. Product Source:E. coli BL21 (DE3) strain expressing a Cas9 gene from Streptococcus pyogenes with an N-terminal 6xHis tag and C-terminal SV40 nuclear localization signal (NLS).Product Includes: Cas9 Nuclease 10x Cas9 Nuclease Reaction Buffer
Apexbio Technology LLC EZ Cap OVA mRNA (m1Ψ) 5x1mg (1mg/mL)
Ovalbumin (OVA) mRNA encodes a egg white glycoprotein with a molecular weight of approximately 45 000 daltons It can induce moderate immunogenicity in organisms and is commonly used as an immunogen in immunological experiments such as establishing animal models for hypersensitivity asthma etc EZ Cap OVA mRNA (m1 ) is provided at 1 mg/mL capped by co-transcriptional capping with a Cap 1 structure and polyadenylated Modified m1 incorporation reduces the host cell immune response and enhances mRNA stability and translation efficiency
Cayman Chemical ANTIBODY KU-0058948 10mg
A PARP1 and PARP2 inhibitor (IC50s = 3.4 and 1.5 nM, respectively); selective for PARP1 over PARP3, PARP4, and tankyrase (IC50s = 40, 1,200, and >10,000 nM, respectively); selectively inhibits the survival of embryonic stem cells lacking Brca1 or Brca2 over embryonic stem cells expressing wild-type Brca1 or Brca2; decreases the survival and induces apoptosis in P39 and MUTZ-3 AML cells at 1 µM; potentiates MS-275-induced cytotoxicity in patient-derived AML cells at 5 nM; reduces survival in PTEN-deficient HCT116 cells but not those expressing wild-type PTEN or HCT116+/neo
Apexbio Technology LLC T4 DNA Ligase (RNase-free)(Synonyms: T4 DNA Ligase, RNase-free T4 DNA Ligase, T4 Ligase, T4 DNA Joining Enzyme), 100KU.
T4 DNA Ligase (RNase-free) is an ATP-dependent enzyme with a molecular weight of approximately 55 3 kDa catalyzing the formation of phosphodiester bonds between adjacent 5 -phosphate and 3 -hydroxyl termini in double-stranded DNA and RNA This enzyme ligates DNA fragments possessing either cohesive or blunt ends and repairs single-strand breaks in double-stranded DNA RNA or DNA/RNA hybrids It is extensively utilized in molecular cloning linker or adaptor ligation nick-repair assays and circularization of linear DNA molecules
Cayman Chemical N-Oleoyl LeucIn 1mg
An N-acyl amide that uncouples mitochondrial respiration independent of UCP1 in vitro; decreases body weight and food intake, preferentially decreasing fat mass in a diet-induced obesity mouse model; improves glucose homeostasis and increases energy expenditure while slightly decreasing overall locomotor activity
Cayman Chemical Oleoyl-L-carnItInchlorIde 5mg
A long-chain acylcarnitine and GlyT2 inhibitor (IC50 = 340 nM); selective for GlyT2 over GlyT1 (IC50 = >10,000 nM); plasma levels are increased in patients with chronic kidney disease or end-stage renal disease receiving incident hemodialysis and these are associated with cardiovascular mortality
Cayman Chemical Octnoyl-Coenzyme AsodIum s 5mg
A medium-chain acyl CoA; levels increased in liver in patients with Reye’s syndrome and β-oxidation of octanoyl-CoA by MCADH is decreased in patients with MCD; inhibits citrate synthase and glutamate dehydrogenase
Cayman Chemical N-Oleoyl LeucIn 5mg
An N-acyl amide that uncouples mitochondrial respiration independent of UCP1 in vitro; decreases body weight and food intake, preferentially decreasing fat mass in a diet-induced obesity mouse model; improves glucose homeostasis and increases energy expenditure while slightly decreasing overall locomotor activity